INFLUENCE OF INTROGRESSION OF AEGILOPS TAUSCHII IN THE WHEAT GENOME ON THE ISOENZYME COMPOSITION AND LIPOXYGENASE ACTIVITY OF SEEDLINGS
Abstract
The isoenzyme composition of lipoxygenase in the seedlings of soft wheat of the Chinese Spring variety and the synthetic hexapoid Synthetic 6x, which carries introgressions of wild grass Aegilops tauschii in its genome, was studied. Synthetic 6x, in comparison with Chinese Spring, had additionally two soluble and one membrane molecular enzyme forms. The activity of lipoxygenase in parents and 7 introgressive lines of Chinese Spring (Synthetic 6x) on chromosome 5D was also examined. Osmotic stress initiated an increase in the activity of the membrane form of the enzyme in the seedlings of the synthetic hexapoid. It is possible that membrane lipoxygenase with a molecular weight of 115 kDa, introduced by Ae. tauschii, participates in the protection of seedlings under osmotic stress. Most of the lines under stress increased the activity of both soluble and membrane-bound lipoxygenase.
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