RESULTS OF PRACTICAL APPLICATION OF COCONUT SUBSTRATE AT THE STAGE OF ADAPTATION OF MICROPLANTS
Abstract
The transfer of plants from in vitro to ex vitro conditions is the final and most stressful stage for microplants, which determines the success of the whole work. At this stage, up to 80% of the planting material often dies, since in vitro plants must adapt to non-sterile ex vitro conditions, which differ significantly from in vitro cultivation conditions (high humidity, carbon dioxide deficiency). The article presents the results of a study of the influence of coconut substrate on the morphological development of microplants of clonal rootstocks of fruit crops obtained by micropropagation. The objects of the study were clonal stocks of American and European selection used for laying intensive and super-intensive orchards – MaxMa14 (cherry), GF677 and Garnem15 (almond, peach, nectarine), Myrobalan 29C (plum, cherry-plum, apricot), OHF87 (pear, quince), Madrasa and Bayan Shirey (local Azerbaijani varieties of grapes). As a result of the study, it was found that, in general, the coconut substrate had a positive effect on the morphological development of regenerated plants. The substrate contributed to the intensification of the growth processes of the aerial and root parts of plants. Rootstock Garnem 15 showed the highest percentage of adapted plants (86,4%). Rootstock GF 677 was distinguished by a good stem formation of 3,8 cm and the longest roots of 3,54 cm. A largest number of formed roots was noted in MaxMa14 regenerated plants – 4,3 pcs.
Full text is available in pdf only
References
Clapa D. Rooting and ex vitro acclimatization in hydroculture by floatation of some blackberry genotypes / D. Clapa, A. Fira, A. Dumitras et al. // The Botanical Review – 2011 – 3(3) – pр. 133–139.
Erfani M. In vitro shoot proliferation and rooting of Garnem rootstock as influenced by basal media, plant growth regulators and carbon sources / M. Erfani, S.M. Miri, A. Imani // Plant Cell Biotechnology and Molecular Biology – 2017 – 18(3&4) – pp. 101–109.
Erturk U. The in vitro rooting performance of pear rootstock 'OHxF 333' in different rooting procedures / U. Erturk // Journal of Food Agriculture and Environment – 2013. – 11(3). – pp. 1424–1427.
Fidancı A. Determination of in vitro propagation techniques of some clonal sweet and sour cherry rootstocks / A. Fidancı, M. Burak, B. Erenoglu et al. // 5th International Cherry Symposium – Bursa – June 06–10, 2005 – р. 409–412.
Fira A. In vitro rooting and ex-vitro acclimation in apple (Malus domestica) / A. Fira, D. Clapa., C. Plopa // Bulletin of University of Agricultural Sciences and Veterinary Medicine – 2010. – 67(1) – p. 480.
Fotopoulos S. In vitro propagation of the peach rootstock: The effect of different carbon sources and types of sealing material on rooting / S. Fotopoulos, T.E. Sotiropoulos // Biologia Plantarum – 2004. – 48(4) – pр. 629–631.
Guney, M. Development of an in vitro micropropagation protocol for Myrobalan 29C rootstock / M. Guney // Turkish Journal of Agriculture and Forestry – 2019 – №43 – pp. 569–575.
Jamwal M. In vitro regeneration of grape (Vitis vinifera L.) cv. Perlette. / M. Jamwal, B. Singh, N. Sharma et al. // World Journal of Agricultural Sciences – 2013 – 9(2) – pp. 161–166.
Mansseri-Lamrioui A. Proliferation and rooting of wild cherry: the influence of cytokinin and auxin types and their concentration / A. Mansseri-Lamrioui, A. Louerguioui, J. Bonaly et al. // African Journal of Biotechnology – 2011 – 10(43) – pp. 8613–8624.
Papakosta E. The in vitro rooting of pear rootstock (Pyrus Communis Pyraster) / E. Papakosta // International Journal of Research in Agricultural Sciences – 2016 – 3(6) – pp. 320–323.
Doroshenko N.P. Osobennosti klonal'nogo mikrorazmnozheniya vinograda [Specifics of clonal micro-propagation of grapes] / N.P. Doroshenko. – Novocherkassk: FSBSI VNIIViV named after Ya.I. Potapenko – 2014. – 204 p. [in Russian]
Krasinskaya T.A. Osnovnye harakteristiki substratov, primenyaemyh v sel'skom hozyajstve [The main characteristics of substrates used in agriculture] / T.A. Krasinskaya, N.V. Kukharchik // Plodovodstvo [Fruit growing] – 2011. – No. 23 – pp. 402–419. [in Russian]
Kukharchik N.V. Razmnozhenie plodovyh rastenij v kul'ture in vitro [Reproduction of fruit plants in culture in vitro] / N.V. Kukharchik, M.S. Kastritskaya, S.E. Semenas et al. // Belarusskaya navuka [Belarusian science] – 2016. – 208 p. [in Russian]
Rundya A.P. Ispol'zovanie ionoobmennyh substratov pri adaptacii rastenij vinograda v usloviyah ex vitro [The use of ion-exchange substrates in the adaptation of grape plants in ex vitro conditions] / A.P. Rundya, O.A. Gashenko, V.A. Shaporeva et al. // Materialy mezhdunarodnoj nauchnoj konferencii "Biotekhnologiya v plodovodstve" [Materials of the international scientific conference "Biotechnology in fruit growing"] – Samachvalavičy, Minsk – June 13–17, 2016 – pp. 128–131. [in Russian]
Shaporeva V.A. Adaptaciya ex vitro podvoya yabloni 54-118 na razlichnyh substratah [Ex vitro adaptation of apple rootstock 54-118 on various substrates] / V.A. Shaporeva, A.A. Zmushko, E.V. Kolbanova // Materialy mezhdunarodnoj nauchnoj konferencii "Biotekhnologiya v plodovodstve" [Materials of the international scientific conference "Biotechnology in fruit growing"] – Samachvalavičy, Minsk – June 13–17, 2016 – pp. 35–39. [in Russian]