QUALITY EVALUATION OF CONIFEROUS SPECIES POLLEN BY STAINING POLLEN GRAINS WITH FLUORESCEIN DIACETATE
Abstract
The results of a comparative study of pollen quality parameters of pine (Pinus sylvestris L.), spruce (Picea abies (L.) Karst. x P. obovata Ledeb.) and juniper (Juniperus communis L.) by in vitro germination and staining pollen grains with fluorescein diacetate are presented. Methods for calculating the pollen quality index of coniferous species differing in the biology of pollen germination are proposed. In 91% of cases, a high (50 < r < 75) or very high (r ≥ 75) positive correlation was observed between the pollen vitality index determined by germination and its merit factor of all studied species. It is shown that the staining of pollen grains with fluorescein diacetate can be used as an express method for assessing pollen of coniferous species' quality.
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