EFFICIENT TECHNIQUE FOR FAST IDENTIFICATION OF SALMONELLA SPP. AND PROTEUS BACTERIAL STRAINS
Abstract
A method for the rapid genotyping of microorganisms using the double digest and selective labeling (DDSL method) was developed to identify strains of pathogenic serotypes of Salmonella spp. and Proteus. Bacterial isolates were identified on species level and grown in liquid media from collected samples ( tissues and bird droppings in different years and from different places). The results of the genotyping of these isolates are consistent with epizootological data, so this method can be recommended for practical use in identifying the routes of infection transmission and localizing the source of pathogens in environment.
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References
Zhebrun A.B. Genotipirovanie i subtipirovanie patogenny`x mikroorganizmov v razvitii texnologij e`pidemiologicheskogo nadzora [Genotyping and subtyping of pathogenic microorganisms in the development of epidemiological surveillance technologies] / A.B. Zhebrun, S.A. Mukomolov, O.V. Narvskaya // Medicinskij akademicheskij zhurnal. – 2009. – №4. – P.59–67. [in Russian]
Tapal`skij D.V. Fenotipicheskoe i molekulyarno-geneticheskoe tipirovanie sal`monell: realii i perspektivy` [Phenotypic and molecular genetic typing of Salmonella: realities and prospects] / D.V. Tapal`skij, V.A. Osipov, S.V. Zhavoronok // Zhurnal mikrobiologii, e`pidemiologii i immunobiologii. – 2005. – №6. – P.88–93. [in Russian]
Dobrina M. N. Nuzhen postoyanny`j kontrol` sal`monelleza [Need constant monitoring of salmonellosis] / M. N. Dobrina // Zhivotnovodstvo Rossii. – 2011. – №3. – P.11-13. [in Russian]
Borisenkova A.N. E`ffektivnost` preparatov razny`x klassov dlya kontrolya sal`monelly` e`nteritidis [The effectiveness of drugs of different classes for the control of salmonella enteritis] / A. N. Borisenkova, O. B. Novikova, M. N. Bajbarak, A. V. Varyuxin // Materialy` XVI konferencii «Dostizheniya v sovremennom pticevodstve: issledovaniya i innovacii». – Sergiev Posad. – 2009. – P.344–347. [in Russian]
Botina S.G. Molekulyarno-geneticheskaya identifikaciya, DNK-genotipirovanie i pasportizaciya molochnokisly`x bakterij roda Lactobacillus [Molecular genetic identification, DNA genotyping and certification of lactic acid bacteria of the genus Lactobacillus] / S.G. Botina // Doklady` na V S``ezde genetikov i selekcionerov. – Moscow. – 2009. – P.49. [in Russian]
Lukinmaa S. Application of molecular genetic methods in diagnostics and epidemiology of food-borne bacterial pathogens / S. Lukinmaa, U-M. Nakari, M. Eklund, A. Siitonen // APMIS. – 2004. – V.112. – P.908–929.
Yakovlev A.F.V. Vliyanie gena gormona rosta na xozyajstvenny`e priznaki pticy [Effect of growth hormone gene on poultry economic traits] / A.F. Yakovlev, V.P. Terleczkij, E`.A. Sekste, L.I. Tuchemskij, Zh.V. Emanujlova // Pticevodstvo. – 2013. – № 1. – P. 2–4. [in Russian]
Terletskiy V. Application and evaluation of double digest selective label (DDSL) typing technique for Pseudomonas aeruginosa hospital isolates / V. Terletskiy, G. Kuhn, P. Francioli, D. Blanc // Journal of Microbiological Methods. –2008. –V.72. – P. 283–287.
Terletskiy V. Validation of Double Digest Selective Label database for sequenced prokaryotic genomes / V. Terletskiy, V. Tyshchenko, I. Martinez-Ballesteros, J.Bikandi, J.Garaizar // Bioinformatics. – 2010. – V. 26. – P.417–418.
Terletski V.P. Subtracted restriction fingerprinting – a tool for bacterial genome typing / V.P. Terletski, S. Schwarz, J. Carnwath, H. Niemann // BioTechniques. – 2003. – V. 34. – P. 304–313.
Hunter P.R. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity / P.R. Hunter, M.A. Gaston // Journal of Clinical Microbiology. – 1988. – V.26. – P.2465–2466.
Bikandi J. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR, and endonuclease restriction / J. Bikandi, R. San Millan, A. Rementeria, J. Garaizar // Bioinformatics. – 2004. – V.22. – P. 798–799.