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Оценка возможностей метода RAPD-PCR для генетической идентификации штаммов B. subtilis
Table 1 - Nucleotide sequences in the primers used for genotyping B. subtilis isolates
Primer | Nucleotide sequence | Т denaturation (15 sec), ºС | Т annealing (15 sec), ºС | Т elongation (60 sec), ºС |
OPA-3 | AGTCAGCCAC | 95 | 37 | 72 |
OPL-12 | GGGCGGTACT | 95 | 37 | 72 |
OPM-15 | GACCTACCAC | 95 | 37 | 72 |
ERIC1 | ATGTAAGCTCCT GGG GATTCAC | 95 | 37 | 72 |
ERIC2 | AAGTAAGTGACT GGG GTGAGCG | 95 | 37 | 72 |
M13 | GAGGGTGGCGGT TCT | 95 | 37 | 72 |