TEMPERATURE DEPENDENCE OF THE FATTY-ACIDS COMPOSITION OF MITOCHONDRIAL MEMBRANES OF PEA SEEDLINGS BEEN TREATING WITH NITROGEN OXIDE DONOR UNDER WATER DEFICIENCY
Abstract
Background: Water deficiency and temperature stress modify cell and organelle membranes, affecting their functions and cell metabolism. In this case, a change in the ratio of unsaturated to saturated fatty acids occurs in the membranes, which leads to a change in the selective permeability of the membranes and the activity of the enzymes associated with them. With the combined action of these factors on the plant organism, synergism in the action of these stressors on the metabolism of plant cells may be observed, or the action of one stressor will reduce the effect of action of another. The exogenous use of signaling molecules or their donors, in particular nitrogen oxide donors, induces plant resistance to stress factors. The aim of the study was to study the fatty acid composition of mitochondrial membranes of etiolated pea seedlings under conditions of insufficient moisture at different temperatures (24o and 17о) and treated with a nitric oxide donor-a tetranitrosyl iron complex with thiosulfate ligands (TNIC-thio).
Methods: The functional state of the mitochondria was studied per the level of lipid peroxidation by the spectrofluorimetry, by a fatty acid composition of mitochondrial membranes with the chromatography technique.
Results: Insufficient moisture led to the activation of lipid peroxidation, which caused changes in the content of C18 and C20 fatty acids (FA). The content of linolenic acid in the membranes of mitochondria of seedlings under water deficiency (WD) and a temperature of 24 ° decreased by 21.3%, and in the membranes of mitochondria of seedlings grown under the same conditions, but at 17 ° - by 13%.Changes occurred in the content of C20 FA: the content of 20: 2 ω6 and 20: 1 ω 9 decreased almost 2.5 times and 1.5 times, respectively (WD + 24o and WD + 17o). The treatment of pea seeds with 10-6M TNIC-thio prevented lipid peroxidation, prevented changes in the composition of the FA membranes of mitochondria and prevented the inhibition of growth of pea seedlings in conditions of water shortage.
Conclusion: It is assumed that a decrease in temperature from 24 ° to 17 ° increases the resistance of pea seedlings to insufficient moisture, which is manifested in less significant changes in the fatty acid composition of mitochondrial membranes i.e. observed changes indicate the presence of cross-adaptation. The antistress activity of the drug is apparently due to the fact that it is a NO donor and is determined by its antioxidant activity.
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References
Vanin A.F. Dinitrozil'nyye kompleksy zheleza s tiol-soderzhashchimi ligandami: fiziko-khimiya, biologiya, meditsina [Dinitrosyl complexes of iron with thiol-containing ligands: physical chemistry, biology, medicine]/A.F. Vanin - M. - Izhevsk: Institute for Computer Research, 2015. -220 p. ISBN 978-5-4344-0276-7 [in Russian].
Zhigacheva I. V. Tetranitrozil'nyybiyadernyy kompleks zheleza povyshayet ustoychivost' prorostkov gorokha i kletok E. coli k stressovym vozdeystviyam [Tetranitrosyl binuclear iron complex increases stress resistance of pea seedlings and E. coli cells] / I.V. Zhigacheva, S.V. Vasilieva, I.P. Generozova and others // Biologicheskiye membrany [Biological membranes]. - 2020.- 37 (2) .- P. 149–155 [in Russian].
Zorov D.B. Mitokhondriya kak mnogolikiy Yanus [Mitochondria as the many faces of Janus] / D.B.Zorov, N.K. Isaev, E.Yu. Plotnikov and others // Biokhimiya [Biochemistry.Moscow]. – 2007. - 72 (10). - P. 1371–1384 [in Russian].
Ivanova T.V. Povyshennoye soderzhaniye zhirnykh kislot s ochen' dlinnoy tsep'yu v lipidakh vegetativnykh organov galofitov [High content of very long chain fatty acids in the lipids of halophyte autonomic organs] / T.V. Ivanova, N.A. Myasoedov, V.P. Pchelkin and others// Fiziologiya rasteniy [Russian J. Plant Physiology]. - 2009.- 56.- P. 871−878 [in Russian].
Kuznetsov Vl. V. Vodonagnetayushchaya aktivnost' kornevoy sistemy pri kross-adaptatsii rasteniy podsolnechnika k gipertermi i vodnomu defitsitu [Water-injecting activity of the root system during the cross-adaptation of sunflower plants to hyperthermia and water deficiency] / Vl. V. Kuznetsov. // Fiziologiya rasteniy. [Russian J. Plant Physiology]. - 2004. - 51 (6).- P. 805-809. [in Russian].
Kuznetsov Vl. V. Kross-adaptatsiya rasteniy kartofelya k deystviyu nizkikh temperatur i zarazheniyu kartofel'noy tsistoobrazuyushchey nematodoy [Cross-adaptation of potato plants to low temperatures and infection by a potato cyst nematode] / Vl. V. Kuznetsov, V.P.Kholodova // Fiziologiya rasteniy [Russian J. Plant Physiology]. - 2011.-58 (6).- P. 803 -804 [in Russian].
Makarenko S.P. Zhirnokislotnyy sostav lipidov membran mitokhondriy Zea mays i Elymus sibiricus [Fatty acid composition of lipids of mitochondrial membranes Zea mays and Elymus sibiricus] / S.P Makarenko, YU.M.Konstantinov, S.V. Khotimchenko and others // Fiziologiya rasteniy [Russian J. Plant Physiology]. -2003.- 50. - P. 548−553 [in Russian].
Mamayeva A.A. Regulyatornaya rol' oksida azota v rasteniyakh [The regulatory role of nitric oxide in plants] / A.A. Mamayeva, A.V. Fomenkov, I.Ye. Nosov and others //Fiziologiya rasteniy [Russian J. Plant Physiology]. - 2015.- 62 (4). - P. 459– 474 [in Russian].
Popov V.N. Vliyaniye ingibitorov elektronnogo transporta na obrazovaniye aktivnykh form kisloroda pri okislenii suktsinata mitokhondriyami gorokha [The influence of electron transport inhibitors on the formation of reactive oxygen species during the oxidation of succinate by pea mitochondria] / V.N. Popov, E.K. Ruge, A.A Starkov// Biokhimiya [Biochemistry.Moscow]. - 2003. - 68 (7). - P.910–916 [in Russian].
Puchkov M. YU.Izucheniye vliyaniya regulyatorov rosta na ovoshchnykh kul'turakh [Studying the influence of growth regulators on vegetables] / M. YU. Puchkov, M. M. M. Abdel'kader// Yestestvennyye nauki. Ekologiya [Natural Sciences. Ecology]. - 2017 - № 1 (58). – P. 13-22 [in Russian].
Sanina N.A. Stroyeniye i svoystva nitrozil'nykh kompleksov zheleza s funktsional'nymi serosoderzhashchimi ligandami [The structure and properties of nitrosyl iron complexes with functional sulfur-containing ligands] / N. A. Sanina, S. M.Aldoshin // Izv. RAN. Ser. Khim [Izv. RAS. Ser. Chem]. – 2011. - № 7. – P. 1199-1227 [in Russian].
Smolina A. V. Antioksidantnaya aktivnost' tetranitrozil'nogo kompleksa zheleza s tiosul'fatnymiligandami i yego vliyaniye na kataliticheskuyu aktivnost' mitokhondrial'nykh fermentov v opytakh [Antioxidant activity of the tetranitrosyl iron complex with thiosulfate ligands and its effect on the catalytic activity of mitochondrial enzymes in experiments] / Smolina A. V. Poletaeva D. A., Soldatova YU. V. and others // DAN [Reports of the Academy of Sciences]. - 2019. – 488 (5). - P. 571-575 [in Russian].
Chirkova T. V. Fiziologicheskiye osnovy ustoychivosti rasteniy [Physiological basis of plant resistance]/ T.V. Chirkova - St. Petersburg: Publishing House of St. Petersburg University, 2002. - 244 p. [in Russian].
Shakirova F.M. Nespetsificheskaya ustoychivost' rasteniy k stressovym faktoram i yeye regulyatsiya [Non-specific resistance of plants to stress factors and its regulation]/ F.M. Shakirova -Ufa: «Gilem», 2001. - 160 p.
Ahmad P. Exogenous application of nitric oxide modulates osmolyte metabolism, antioxidants, enzymes of ascorbate-glutathione cycle and promotes growth under cadmium stress in tomato/ P. Ahmad, M.A. Ahanger, M.N. Alyemeni et al. // Protoplasmaю - 2018. -. 255. - P. 79-93. DOI:
Ainsworth E.A. Targets for crop biotechnology in a future high-CO2 and high-O3 world/ E.A. Ainsworth, A. Rogers, A.D.B Leakey // Plant Physiol. – 2008 – 147.- 13-19.
Atkin O.K. The crucial role of plant mitochondria in orchestrating drought tolerance/ O.K. Atkin, D.Macherel// Annals of Botany. – 2009. -103 - P. 581-597.
Bertolli S. C. The level of environmental noise affects the physiological performance of Glycine max under water deficit. / S. C Bertolli, G. M. Souza // Theor. Exp. Plant Physiol. – 2013. -.25 (1). – P. 416
Carreau J.P. Adaptation of Macroscale Method to the Microscale for Fatty Acid Methyl Trans esterification of Biological Lipid Extracts/ J.P Carreau., J.P Dubacq // J. Chromatogr.- 1979. - 151. – P. 384−390.
Fletcher B.I. Measurement of fluorescent lipid peroxidation products in biological systems and tissues / B.I. Fletcher, C.D. Dillard., A.L.Tappel // Anal. Biochem.-1973. - 52. - P. 1–9
Fahad Sh. Exogenously Applied Plant Growth Regulators Enhance the Morpho-Physiological Growth and Yield of Rice under High Temperature/Shah Fahad, Saddam Hussain, Shah Saud, et al.// Front. Plant Sci.- 2016. - 7(7). – P. 1250.
Generozova I.P. Mitochondrial respiration after combined action of dehydration and low temperature in pea seedlings/ I.P. Generozova., P.A. Butsanets, A.G Shugaev // Biologia Plantarum. – 2019. – 63.-P. 11- 19.
Golovina R.V. Thermodynamic Evalu!ation Interaction of Fatty Acid Methyl Esters with Polar and Nonpolar Stationary Phases, Based on Their Retention Indices Chromatographia/ R.V. Golovina, T.E. Kuzmenko // Chromatography. - 1977. - 10. - P. 545−546.
Groß F. Nitric oxide, antioxidants and prooxidants in plant defence responses/ F.Groß, J. Durner, F. Gaupels // Front Plant Sci.- 2013.- 4.- P. 419.
Guo Yun-ping, Li Jia-rui (2002). Changes of fatty acids composition of membrane lipids, ethylene release and lipoxygenase activity in leaves of apricot under drought / Guo Yun-ping, Li Jia-rui // Journ.of Zhejiang University (Agrical & Life Sci). – 2002. - 28(5). - P. 513-517.
Lakomkin V.L.Long-lasting hypotensive action of stable preparations of dinitrosyl-iron complexes with thiol-containing ligands in conscious normotensive and hypertensive rat/ V.L. Lakomkin, A.F. Vanin, A.A. Timoshin et al // Nitric Oxide. - 2007. -16 (4).- P.413-418
Lambers H. Plant nutrient-acquisition strategies change with soil age/ H. Lambers, J.A Raven, G.R. Shaver et al // Trends Ecol. Evol.- 2008.- 23. - P. 95-103
Le Gall H. Cell Wall Metabolism in Response to Abiotic Stress/ H Le Gall, F. Philippe, J.M.,Domon et al.// Plants (Basel).- 2015. - 4(1). – P.112-166.
Marvasi M. Use and perspectives of nitric oxide donors in agriculture /M.Marvasi // Journal of the Science of Food and Agriculture. - 2017. - 97 (4). - P. 1065-1072.
Nabi R. B. S.. Nitric oxide regulates plant responses to drought, salinity, and heavy metal stress / R. B. S. Nabi, R. Tayade, A. Hussain et al. // Environmental and Experimental Botany. - 2019.- 161.- P. 120-133
Okçu G.. Effects of Salt and Drought Stresses on Germination and Seedling Growth of Pea (Pisum sativum L.) / G. Okçu, M.D.Kaya, M. Atak // Turk J Agric For.- 2005.-29.- P. 237-242.
Peng M. The Effect of Plant Growth Regulator and Active Charcoal on the Development of Microtubers of Potatoes / M. Peng, X. Wang, L. Li// AJPS. – 2012.-3(11). – P. 1535-1540
Raffaele S. A MYB transcription factor regulates very-long chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis / S. Raffaele, F.Vailleau, A Léger.et al. //Plant Cell.- 2008.- 20.- P. 752–767.
Seabra A. B .How nitric oxide donors can protect plants in a changing environment: what we know so far and perspectives / A. B. Seabra, H. C. Oliveira // AIMS Molecular Science. - 2016. - 3(4). - P. 692-718
Vladimirov Y.A. Lipid peroxidation in mitochondrial membrane/ Y.A. Vladimirov , V.I. Olenev, T.B. Suslova// Adv Lipid Res.- 1980, -17. - P.173-249.
Wang J. Fatty Acid Determination in Chicken Egg Yolk.A Comparison of Different Methods/ J. Wang, H.Sunwoo, G. Cherian et al// Poultry. Science. - 2000. – 79. – P.1168−1171.
Yue Niu. An Overview of Biomembrane Functions in Plant Responses to High-Temperature Stress/ Yue Niu, Yun Xiang// Front. Plant Sci. – 2018. - 9. – P. 915-935.