INFLUENCE OF THE REACTIVE OXYGEN SPECIES AND IONIZING RADIATION ON YEAST CELLS SACCHAROMYCES CEREVISIAE AND SACCHAROMYCES CARLSBERGENSIS UNDER ILLUMINATION BY VISIBLE LIGHT
Abstract
In this present work the yeast Saccharomyces cerevisiae and Saccharomyces Carlsbergensis against oxidizing stress as a result of exposure includes growing of yeast culture under standard conditions till the end of the logarithmic or the start of the stationary phase of growth, incubation with a protective agent. The protective agent is represented by inhibitor - naphthalene C10H8 in concentration of 5,10-4 M. Naphthalene is a part of heavy oil fractions and is released into the environment as a result of accidents, during the combustion of various fuels, it is found in the exhaust gases of cars and is classified as a carcinogen and mutagen. Dose-effect curves were obtained when exposed to ionizing radiation on yeast Saccharomyces cerevisiae and Saccharomyces carlsbergensis cells, the effect of additives (hydrogen peroxide, ethanol, naphthalene) on the growth and viability of yeast in the post-radiation period was evaluated, and the effect of visible light on the growth and viability of cells after irradiation was investigated.
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