АНТИОКСИДАНТЫ ПОВЫШАЮТ УСТОЙЧИВОСТЬ ПРОРОСТКОВ ГОРОХА К ДЕФИЦИТУ ВОДЫ
Table 1 - The rates of NAD-dependent substrate oxidation by pea seedling mitochondria, as affected by water deficiency and the tested antioxidants
Group | V2 | V3 | V4 | V3/V4 | FCCP |
Control | 22.90±1.20 | 71.40±1.40 | 31.32±1.10 | 2.28±0.01 | 72.80±1.80 |
WD | 15.00±1.10 | 42.84±2.00 | 26.60±1.00 | 1.61±0.02 | 41.10±2.00 |
WD+AMB | 24.74±1.40 | 76.50±1.20 | 33.00±1.25 | 2.32± 0.01 | 77.40±2.10 |
WD+CAR | 25.14±1.21 | 80.45±1.90 | 32.19±1.52 | 2.50±0.01 | 74.65±1.75 |
WD+RES | 18.76±1.00 | 69.60±1.31 | 31.64±1.40 | 2.20±0.02 | 70.22±2.00 |
rates: ng O2 atom/mg protein × min (n=10). Incubation medium: 0.4 M sucrose, 20 mM HEPES-Tris buffer (pH 7.2), 5 mM KH2PO4, 4 mM MgCl2, 10 mM malate, 10 mM glutamate. Additional additives: 200 μM ADP, 10-6 M FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone). Legend: V2 – rates of substrate oxidation, V3 – rates of substrate oxidation in the presence of ADP; V4 – oxidation rate under resting conditions (rates of substrate oxidation when ADP is exhausted)