GROWTH OF ISOCHRYSIS GALBANA IN MIXOTROPHIC CONDITIONS USING GIBBERELLIC ACID

Main Article Content

N.N. Kovalev
S.E. Leskova
E.V. Miheev

Abstract

The study of influence of different concentrations of gibberellic acid on growth of Isochrysis galbana in enrichment culture has been conducted. The growth of algae biomass was found by increasing the number of cells calculated in each experiment in three hemocytometers under a light microscope. The duration of the experiment was 8 days. Concentrations of gibberellic acid of 0.39 × 10-8 and 0.79 10-8mmol/lt were found to have a stimulating effect on cell growth dynamics of I. galbana, compared to the control group. The maximum cell growth in the culture was observed when gibberellic acid was added to concentrations of 0.39 ×10-8 mmoll/lt and was 721%. Biochemical indicators of algae culture of I. galbana were evaluated, with the addition of gibberellic acid in concentrations of 0.39 ×10-8 mml during 8 days of the experiment in comparison with the control group. Compared to the control group, an increase in the amount of protein, lipids, carbohydrates and chlorophyll in the culture of algae was established with the addition of gibberellic acid at a concentration of 0.39 ×10-8 mml at the time of the experiment.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Article Details

Section
Auxiliary disciplines

References

Румянцев Д.Е. Диагностика особенностей роста сосны и ели в Южной Карелии с использованием методов дендрохронологии. Автореф дис...канд. биол. наук. / Д. Е. Румянцев. – М.– МГУЛ – 22 с.

Fritts H.C. Tree rings and climate / H.C. Fritts. – London – New York – San Francisco – Academic press – 1976 – 576 p.

Соломина О.Н. Засухи Восточно-Европейской равнины по гидрометеорологическим и дендрохронологическим данным / О. Н. Соломина, И.С. Бушуева, Е.А. Долгова и др. – СПб. – Нестор-История. – 2017 – 360 с.

Чендев Ю.Г. Почвы и растительность юга Среднерусской возвышенности в условиях меняющегося климата / Ю. Г. Чендев, М.Г. Лебедева, С.М. Матвеев и др. – Белгород, Константа – 2016 – 326 с.

Cook E.A. A time series analysis approach to tree ring standartization. A dissertation Submitted to the Faculty of the School of renewable natural resources. / E.A. Cook – University of Arizona – 1985 – 171 p.

Matveev S.M. Climatic changes in the East-European forest-steppe and effects on Scots pine productivity / S. M. Matveev, Yu.G. Chendev, A. R. Lupo et al. // Pure and Applied Geophysics – 2017 – V. 174 – №1 – pp. 427–443

Матвеев С.М. Дендрохронология. / С. М. Матвеев – Воронеж – ВГЛТА – 2001 – 88 с.

Lovelius N.V. Dendroindication of natural processes and antropogenic influences / N.V. Lovelius – St-Peterburg, 1997 – 320 p.