ВЛИЯНИЕ АКТИВНЫХ ФОРМ КИСЛОРОДА И ИОНИЗИРУЮЩЕГО ИЗЛУЧЕНИЯ НА ДРОЖЖЕВЫЕ КЛЕТКИ SACCHAROMYCES CEREVISIAE и SACCHAROMYCES CARLSBERGENSIS ПРИ ОСВЕЩЕНИИ ВИДИМЫМ СВЕТОМ
Аннотация
В настоящей работе защита дрожжей Saccharomyces cerevisiae и Saccharomyces Carlsbergensis от окислительного стресса в результате воздействия включает выращивание дрожжевой культуры в стандартных условиях до конца логарифмической или начала стационарной фазы роста, инкубацию с защитным агентом. Защитное средство представлено ингибитором - нафталином C10H8 в концентрации 5,10-4 М. Нафталин входит в состав тяжелых нефтяных фракций и выделяется в окружающую среду в результате аварий, при сгорании различных видов топлива, он содержится в выхлопных газах автомобилей и классифицируется как канцероген и мутаген. Были получены кривые доза-эффект при воздействии ионизирующего излучения на клетки дрожжей Saccharomyces cerevisiae и Saccharomyces carlsbergensis, оценено влияние добавок (перекись водорода, этанол, нафталин) на рост и жизнеспособность дрожжей в пострадиационный период, а также влияние видимого света на рост и жизнеспособность дрожжей. исследовали клетки после облучения.
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