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The material presents data on the identification of bacterial strains isolated from turkeys and chicken. We used isolates of Pseudomonas, Acinetobacter and Escherichia coli. The latter were isolated from turkeys, and two previous ones from chickens. In-silico search made it possible to propose a number of enzymes for genotyping isolates of Acinetobacter, and genotyping of isolates of Pseudomonas and E. coli was carried out using previously selected enzymes. The enzymes SgsI and Eco47III, which show 100% activity in one buffer, have been shown to have optimal combination for Acinetobacter. Pseudomonas were genotyped using a pair of BcuI/StuI enzymes, two genetically different strains were identified, two isolates were identical (organs of one individual). In 21 isolates of E.coli, 17 strains were identified using the XbaI/PstI pair of enzymes, and 16 strains were identified using the XhoI/BsuRI pair. The discrimination index showing the ability of the method to separate strains was not used, since it can only be used on isolates that are not epidemiologically related. In this case, turkeys were kept in the same farm facilities, the sampling was carried out at the same time, therefore, the possibility of re-infection of the bird cannot be ruled out.
2. Terletskiy V.P. Effektivnyj molekulyarno-geneticheskij metod identifikaczii shtammov salmonell i proteya [The efficient molecular genetic technique for identification of Salmonella and Proteus strains] / V.P. Terletskiy, V.I. Tyshchenko, O.B. Novikova, A.N. Borisenkova, D.E. Belash, A.F. Yakovlev // Russian Agricultural Sciences. – 2013. – No 5. – P. 60-63.
3. Bikandi J. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR, and endonuclease restriction / J.Bikandi, R.San Millán, A. Rementeria, J. Garaizar // Bioinformatics. – 2004. – V. 22. – P. 798-799.
4. Gibson J.R. Comparison of PFGE, ribotyping and phage-typing in the epidemiological analysis of Campylobacter jejuni serotype HS2 infections / J.R. Gibson, C. Fitzgerald, R.J. Owen // Epidemiol. Infect. – 1995. – V. 115. – No 2. – P. 215-225.
5. Lartigue M.F. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for bacterial strain characterization / M.F. Lartigue // Infect. Genet. Evol. – 2013. – V. 13. – P. 230-235.
6. Meisel S. J. Raman Spectroscopy as a Potential Tool for Detection of Brucella spp. in Milk / S. Meisel, S. Stöckel, M. Elschner, F. Melzer, P. Rösch, J. Poppa // Appl. Environ. Microbiol. – 2012. – V. 78. – No16. – P. 5575-5583.
7. Minharro S. Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008 / S. Minharro J.P. Silva, E.M. Dorneles, R.B. Pauletti, H. Neubauer, F. Melzer, F.P. Poester, M.G. Dasso, E.S. Pinheiro, P.M. Soares, R.L. Santos, M. Heinemann A.P. Lage // PLoS ONE. – 2013. – V.8. – No12: e81152. doi:10.1371/journal.pone.0081152.
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