MOLECULAR GENETIC IDENTIFICATION OF BACTERIAL STRAINS OF THREE SPECIES ISOLATED FROM TURKEY AND CHICKEN

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Valery Pavlovich Terletskiy
Oksana Borisovna Novikova
Valentina Ivanovna Tyshchenko
Lydiya Aleksandrovna Shinkarenko

Abstract

The material presents data on the identification of bacterial strains isolated from turkeys and chicken. We used isolates of Pseudomonas, Acinetobacter and Escherichia coli. The latter were isolated from turkeys, and two previous ones from chickens. In-silico search made it possible to propose a number of enzymes for genotyping isolates of Acinetobacter, and genotyping of isolates of Pseudomonas and E. coli was carried out using previously selected enzymes. The enzymes SgsI and Eco47III, which show 100% activity in one buffer, have been shown to have optimal combination for Acinetobacter. Pseudomonas were genotyped using a pair of BcuI/StuI enzymes, two genetically different strains were identified, two isolates were identical (organs of one individual). In 21 isolates of E.coli, 17 strains were identified using the XbaI/PstI pair of enzymes, and 16 strains were identified using the XhoI/BsuRI pair. The discrimination index showing the ability of the method to separate strains was not used, since it can only be used on isolates that are not epidemiologically related. In this case, turkeys were kept in the same farm facilities, the sampling was carried out at the same time, therefore, the possibility of re-infection of the bird cannot be ruled out.

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How to Cite
TERLETSKIY, Valery Pavlovich et al. MOLECULAR GENETIC IDENTIFICATION OF BACTERIAL STRAINS OF THREE SPECIES ISOLATED FROM TURKEY AND CHICKEN. Journal of Agriculture and Environment, [S.l.], n. 4 (16), dec. 2020. ISSN 2564-890X. Available at: <http://jae.cifra.science/article/view/233>. Date accessed: 21 jan. 2021. doi: http://dx.doi.org/10.23649/jae.2020.4.16.5.
Section
Animal husbandry
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