LACTOGENIC EFFECTS OF PROBIOTIC PREPARATION ON THE BASE OF RECOMBINANTLACTOBACILLI WITH GROWTH HORMONE RELEASING FACTOR GENE IN RUMINANTS

Main Article Content

Zinoviy Nikolaevich Makar

Abstract

For the first time, lactogenic action of parenteral administration of the extract from the pituitary gland was detected in Russia (Azimov, Krouze, 1937). However, stimulation of milk production with the help of growth hormone injections is associated with a number of shortcomings. The aim of this work was to study the effects of oral administration of genetically engineered recombinant strain of Lactobacillus sp. 8 RAZ (pLF-SL2), Reg. No. В-7495, expressing synthesis of growth hormone releasing factor (GH-RF) in digestive tract, to enhance the milk production in ruminants. In the experiment I, conducted on goats, the recombinant preparation was given as feed additive to 5 goats on the 3rd month of lactation daily for 6 weeks at a dose of 25109 CFU per day, 5 goats served as control. In experiment II, a recombinant preparation was given to 8 cows at 4-5 months of lactation at a dose of 26109 CFU per day for 2 months (group 1), the original non-recombinant strain was given to 8 cows at the same dose (group 2), 8 cows fed basic diet served as control. Experiment III was carried out on three groups of cows at the 3-4th month of lactation, 10 cows each. The cows of group 1 were fed a recombinant probiotic daily at a dose of 200109 CFU per day, and group 2 at a dose of 1000109 CFU daily. The control group of cows received only the basic diet. In all experiments, the recombinant preparation stimulated milk production and increased the conversion of feed into milk components after three-week lag period. In goats of experimental group, the daily milk yield (DMY) during the last 2-3 weeks was higher by 23.5% (P<0.05) than in control group. In experiment II, during the last 5 weeks, DMY in group 1 was more by 10% (P<0.05) compared to group 2 and by 8.1% (P<0.05) compared to group 3. In experiment III, DMY in groups 1 and 2 was higher by 6.3 and 13.2% (P<0.05), respectively, in comparison with control. The data obtained show that the use of recombinant strains of lactic acid bacteria expressing GH-RF may be an alternative to the use of injections of growth hormone in order to increase the milk productivity and quality of milk in ruminants.

Article Details

How to Cite
MAKAR, Zinoviy Nikolaevich. LACTOGENIC EFFECTS OF PROBIOTIC PREPARATION ON THE BASE OF RECOMBINANTLACTOBACILLI WITH GROWTH HORMONE RELEASING FACTOR GENE IN RUMINANTS. Journal of Agriculture and Environment, [S.l.], n. 4 (12), nov. 2019. ISSN 2564-890X. Available at: <http://jae.cifra.science/article/view/183>. Date accessed: 31 mar. 2020. doi: http://dx.doi.org/10.23649/jae.2019.4.12.18.
Section
Animal husbandry
References
Azimov G.I. The lactogenic preparations from the anterior pituitary and the increase of milk yield in cows / Azimov G.I., Krouze N.K. // J. Dairy Sci. – 1937.-. V. 20. P. 289−306.
Bauman D.E. Responses of high-producing dairy cows to long-term treatment with pituitary somatotropin and recombinant somatotropin / Bauman D.E., Eppard P.J., Degeeter M.J., Lanza G.M. // J. Dairy Sci. – 1985. – V. 68. – P. 1352-1362.
Johnsson I. D. I. Manipulation of milk yield with growth hormone / Johnsson I. D. I., Hart C. In: Recent Advances in Animal Nutrition (Eds W. Haresign, D.J.A. Cole). London: Butterworth. – 1986 - 105-121p.
Bauman D.E. Bovine somatotropin and lactation: From basic science to commercial application / Bauman D.E. // Domest. Anim. Endocrinol. – 1999. - V. 17. - P. 101–116.
Tucker H.A. Hormones, mammary growth, and lactation: a 41-year perspective /./ Tucker H.A // J. Dairy Sci. – 2000. – V. 83. – P. 874-884.
Dohoo I.R. A meta-analysis review of the effects of rbSTH. 1. Methodology and effects on production and nutrition related parameters / Dohoo I.R., Leslie K.E., Des Côteaux L. // Can. J. Vet. Res. - 2003. – V.67. – P. 241–251.
Akers R.M. Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows / Akers R.M. // J. Dairy Sci.. – 2006. – V. 89. – P. 1222-1234.
Makar Z.N. [Effect of exogenous somatotropin on blood supply and secretory activity of the mammary gland in cows] / Makar Z.N. // Problemy biologii productivnykh zhivotnykh – [Problems of Productive Animal Biology] - 2012. - No3. – P. 36-43.[In Russian]
Makar Z.N. Regulyatsiya krovosnabzheniya i funktsional’noi aktivnosti molochnoi zhelezy u zhvachnykh zhivotnykh [Regulation of blood supply and functional activity of the mammary gland in ruminants]. // Extended Abstract of Diss. Dr. Sci. Biol., Borovsk, 2012. - 48 p. [In Russian]
Dahl G.E. Galactopoietic effects of recombinant somatotropin and growth hormone-releasing factor in dairy cows / Dahl G.E., Chapin L.T., Moseley W.M., Tucker H.A. // J. Dairy Sci. – 1993. – V. 76. - P 1550-1557.
Shingu H. Effects of growth hormone-releasing hormone treatment on milk production and plasma hormones and metabolites in lactating Japanese Black cows under negative energy balance / Shingu H., Hodate K., Kushibiki S. et al. // J. Anim. Sci. – 2009. – V. 87. – P. 1247-1253.
Webb K.E. Intestinal absorption of protein hydrolysis products: a review / Webb K.E. // J. Anim. Sci. – 1990. – V.68. – P. 3011-3020.
Matthews D.M. Protein Absorption: Development and Present State of the Subject. New York: Wiley-Liss, Inc., 1991.
Tarakanov B.V. [Biological evaluation of the effect on the body of rabbits of recombinant strains of lactobacilli with the growth hormone releasing factor gene] / Tarakanov B.V., Nikolicheva T.A., Manukhina A.I., Aleshin V.V. // Agricultural Biology – 2003 – No 4. - V. P. 10-20. [In Russian]
Aleshin V.V. [The cloning of the GH-RF gene in lactic acid bacteria and its expression from the replicative vector gene promoter]. / Aleshin V.V., Semenova E.V., Tarakanov B.V., Karpushina S.G. // In: Modern problems of biotechnology and biology of productive animals. Borovsk: VNIIFBiP Publ. – 1999 – V. 38. – P. 116-129. [In Russian]
Tarakanov B.V. [Prospects for the creation of new probiotics based on recombinant strains of bacteria expressing eukaryotic genes] / Tarakanov B.V., Ernst L.K. Moscow: Rosselkhozakademiya Publ. – 2002. – 71. - p. [In Russian]
Etherton T.D. Biology of somatotropin in growth and lactation of domestic animals / Etherton T.D., Bauman D.E. // Physiol. Rev. – 1998. – V. 78. – P. 745-761.
Baldi A. Bovine somatotropin administration to dairy goats in late lactation: effects on mammary gland function, composition and morphology ./ Baldi A., Modina S., Cheli F., Gandolfi F. et al. // J. Dairy Sci. – 2002. – V. 85. - P. 1093-1102.
Chiado S. Effects of recombinant bovine somatotropin administration to lactating goats / Chiado S., Gzervas K., Kiriakou K. // Small Rum. Res. – 2000. – V. 35. - P. 263-269.
Gardner M.L.G. Intestinal assimilation of intact peptides from the diet – a neglected field? / Gardner M.L.G. // Biological Reviews. – 1984. – V. 59. – P. 289-300.
Tarakanov B.V. [Using probiotics in animal husbandry] / Tarakanov B.V. – Kaluga: Department of Agriculture and Food, Publ. – 1998. – 58 p. [In Russian]