PCR IDENTIFICATION AND CHARACTERIZATION OF ENZYMATIC ACTIVITY AND ANTAGONISTIC PROPERTIES OF BACTERIA OF THE GENUS PSEUDOMONAS ISOLATED FROM NATURAL SOURCES

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Elena I. Ladutska

Abstract

The molecular genetic identification of bacterial strains of genus Pseudomonas isolated from soil and vegetative material of potato plants (Solanum tuberosum) was carried out. PCR analysis using the genus-specific OpriF / OpriR primer showed that 9 samples of a pure bacterial culture were characterized by the presence of one amplicon characteristic of Pseudomonades. Affiliation of selected bacteria to genus Pseudomonas was confirmed by a comparative analysis of the deciphered nucleotide sequence of the 16S rRNA gene with the reference nucleotide sequences of typical strains from the RDP database. A wide range of enzymatic activity and pronounced antagonistic properties in relation to phytopathogenic micromycetes of genera Alternaria and Fusarium were established in selected strains of bacteria.

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How to Cite
LADUTSKA, Elena I.. PCR IDENTIFICATION AND CHARACTERIZATION OF ENZYMATIC ACTIVITY AND ANTAGONISTIC PROPERTIES OF BACTERIA OF THE GENUS PSEUDOMONAS ISOLATED FROM NATURAL SOURCES. Journal of Agriculture and Environment, [S.l.], n. 2 (10), july 2019. ISSN 2564-890X. Available at: <http://jae.cifra.science/article/view/153>. Date accessed: 18 sep. 2019. doi: http://dx.doi.org/10.23649/jae.2019.2.10.8.
Section
Crop production
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