APPLICATION OF DNA TYPING FOR IDENTIFICATION OF BACTERIAL STRAINS INFECTING ANIMALS

Main Article Content

Valery Pavlovich Terletskiy
Valentina Tyshchenko
Magomed Gaplaev

Abstract

Typing of an important pathogen, Staphylococcus aureus, permits epidemiological investigations within a community and within an individual hospital. Pulsed field gel electrophoresis (PFGE) is the current gold standard method for typing this bacterial species in short-term epidemiological studies. However, PFGE requires specialized equipment and is rather slow. This article demonstrates the potential of a new typing method  (double digest selective label DDSL) as a fast, highly discriminatory alternative to PFGE for the typing of Staphylococcus aureus isolates. In this new typing method, large DNA fragments are produced with a restriction enzyme commonly used for PFGE but are trimmed by a second enzyme to a size which can be separated on a conventional agarose gel within a short period of time. Selective labelling of a subset of the numerous restriction fragments gives a distinct banding pattern for each isolate. The advantages of DDSL typing are its speed of analysis (one day) and the potential for greater discrimination between isolates than can be achieved with PFGE typing. An online service allows simulating the technique and a suggestion tool is developed for easy selection of endonucleases for this genotyping method.

Article Details

How to Cite
TERLETSKIY, Valery Pavlovich; TYSHCHENKO, Valentina; GAPLAEV, Magomed. APPLICATION OF DNA TYPING FOR IDENTIFICATION OF BACTERIAL STRAINS INFECTING ANIMALS. Journal of Agriculture and Environment, [S.l.], n. 1 (9), apr. 2019. ISSN 2564-890X. Available at: <http://jae.cifra.science/article/view/125>. Date accessed: 20 may 2019. doi: http://dx.doi.org/10.23649/jae.2019.1.9.9.
Section
Animal husbandry
References
Ross T.L. Comparison of an automated repetitive sequence-based PCR microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus / T.L. Ross, W.G. Merz, M. Farkosh and others // J. Clin. Microbiol. – 2005. – Vol. 43. – P. 5642–5647.
Blanc D.S. The use of molecular typing for epidemiological surveillance and investigation of endemic nosocomial infections / D.S. Blanc // Infect. Genet. Evol. –2004. – Vol. 4. – P. 193–197.
Strandén, A., R. Frei, A.F. Widmer. Molecular typing of methicillin-resistant Staphylococcus aureus: can PCR replace pulsed-field gel electrophoresis? / A. Strandén, R. Frei, A.F. Widmer // J. Clin. Microbiol. – 2003. – Vol. 41. – P. 3181–3186.
Trindade P.A. Molecular techniques for MRSA typing: current issues and perspectives / Trindade, P.A., J.A. McCulloh, G.A. Oliveira and others // Braz. J. Infect. Dis. – 2003. – Vol. 7. – P. 32–43.
Mitani N. Molecular typing of methicillin-resistant Staphylococcus aureus by PCR-RFLP and its usefulness in an Epidemiological study of an outbreak / N. Mitani, A. Koizumi, R. Sano and others // Jpn. J. Infect. Dis. – 2005. –Vol. 58. – P. 250–252.
Koort J.M.K. Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis / J.M.K. Koort, S. Lukinmaa, M. Rantala and others // J. Clin. Microbiol. – 2002. – Vol. 40. – P. 3497–3498.
Fakhr M.K. Multilocus sequence typing lacks the discriminatory ability of pulsed-field gel electrophoresis for typing Salmonella enterica serovar Typhimurium / M.K. Fakhr, L.K. Nolan, C.M. Logue // J. Clin. Microbiol. – 2005. – Vol. 43. – P. 2215–2219.
Foxman B. Choosing an appropriate bacterial typing technique for epidemiological studies / B. Foxman, L. Zhang, J.S. Koopman and others // Epidemiol. Perspect. Innov. – 2005. –Vol. 2. – P.10-17.
Terletski V.P. Subtracted restriction fingerprinting – a tool for bacterial genome typing / V.P. Terletski, S. Schwarz, J. Carnwath and others // BioTechniques. – 2003. – Vol. 34. – P. 304–313.
Strommenger B. Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and their relationship to human isolates / B. Strommenger, C. Kehrenberg, C. Kettlitz and others // J. Antimicrob. Chemother. – 2006. – Vol. 57. – P. 461–465.
Bikandi J. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR, and endonuclease restriction / J. Bikandi, R. San Millán, A. Rementeria and others // Bioinformatics. – 2004. – Vol. 22. – P. 798–799.
Lange K. Molecular subtyping of Staphylococcus aureus isolates from cases of bovine mastitis in Brazil / K. Lange, M. Cardoso, D. Senczek, S. Schwarz // Vet. Microbiol. 1999. – Vol. 67. – P. 127–141.