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Alexey Mikhailovich Osintsev
Vladimir Ilyich Braginsky


Understanding the role of calcium in colloidal stability of casein micelles, that is unquestionable, could become the key to control the process of milk coagulation. It is evident that calcium ions can influence milk coagulation, but the molecular mechanism of this influence to micellar casein system is not fully understandable. Methodologically, our research was based on an idea that calcium ions can change the electric charge of casein micelles in the process of dissociation and recombination of some kinds of phosphoproteins, which are components of the casein micelles. A simple quantitative model, which includes kinetic description of the proteolysis process and the thermodynamics of the dissociation process of the functional groups of micellar caseins, was worked out to analyze experimental results. Kinetic and thermodynamic methods of describing the process of stability loss in micellar system were combined in one model, using the concept of solvent quality which is defined by the second osmotic virial coefficient. Our experiments showed that calcium ions are able to connect chemically to caseins in the micelles. Using reasonable assessments for thermodynamic and kinetic parameters, we managed to get quite adequate description of the experimental data. We also demonstrated principal possibility of using our model to describe rennet, acid and mixed acid-rennet clotting of milk as well as heat-calcium and heat-acid coagulation of milk.

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OSINTSEV, Alexey Mikhailovich; BRAGINSKY, Vladimir Ilyich. PHOSPHOPROTEINS AS A FACTOR FOR COLLOID STABILITY OF CASEIN MICELLES IN MILK. Journal of Agriculture and Environment, [S.l.], n. 1 (9), may 2019. ISSN 2564-890X. Available at: <>. Date accessed: 20 may 2019. doi:
Handling, transporting, storage and protection of agricultural products
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